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Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of the rEPC1. The gel was Coomassie Brilliant Blue stained, and the expected molecular mass of the His6-tagged rEPC1 protein was 12.8 kDa. M = protein marker; lane 2 = Escherichia coli BL21 (DE3) lysates with isopropyl-β-D-thiogalactoside 1 mM after 5 hours of induction; lanes 1 and 3 = rEPC1protein extracts.
