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. 2016 Jun 1;94(6):1318–1323. doi: 10.4269/ajtmh.15-0829

Figure 2.

Figure 2.

(A) Loop-mediated isothermal amplification (LAMP) results and (B) sensitivity of LAMP method with Taenia solium cox1 primer set. LAMP products were separated on a 2.5% agarose gel. Lane M, 100 bp DNA ladder (Nacalai Tesque, Kyoto, Japan). (A) Lane 1, negative control without DNA; lane 2, plasmid for T. solium cox1 gene; lane 3, plasmid for Taenia saginata cox1 gene; and lane 4, plasmid for Taenia asiatica cox1 gene. Tsol-cox1, results of LAMP with primer set from T. solium cox1 gene; Tsag-cox1, results of LAMP with primer set from T. saginata cox1 gene; Tasi-cox1, results of LAMP with primer set from T. asiatica cox1 gene. (B) The standard plasmids were serially diluted from 106 copies to one copy per reaction. Lane 1, negative control and lanes 2–8 represent 106, 105, 104, 103, 102, 10, and one copy (ies)/reaction, respectively.