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. 2016 Jun 1;94(6):1318–1323. doi: 10.4269/ajtmh.15-0829

Figure 3.

Figure 3.

(A) Results of multiplex loop-mediated isothermal amplification (mLAMP) with standard plasmid carrying cox1 gene of each Taenia species and (B) dot enzyme-linked immunosorbent assay (dot-ELISA). (A) The mLAMP products were separated on a 2.5% agarose gel. Lane M, 100 bp DNA ladder (Nacalai Tesque, Kyoto, Japan); lane 1, negative control without DNA; lane 2, standard plasmid for Taenia solium cox1 gene; lane 3, standard plasmid for Taenia saginata cox1 gene; and lane 4, standard plasmid for Taenia asiatica cox1 gene. (B) The mLAMP product from each Taenia species was incubated with antibodies for fluorescein isothiocyanate (FITC), digoxigenin (DIG), tetramethylrhodamine (TAMRA), and mouse IgG immobilized on the nitrocellulose membrane. Row a, FITC antibody; row b, DIG antibody; row c, TAMRA antibody; and row d, mouse IgG. Lane 1, negative control of mLAMP reaction without DNA; lane 2, mLAMP products from plasmid for the T. solium cox1 gene; lane 3, mLAMP products from plasmid for the T. saginata cox1 gene; and lane 4, mLAMP products from plasmid for the T. asiatica cox1 gene.