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. 2016 Jun;137(6):1907–1909. doi: 10.1016/j.jaci.2016.01.030

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© 2016 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig 2 — Treg-cell–intrinsic amphiregulin is not required for suppressive function in vitro. A, Confirmation of genotype by quantitative RT-PCR. Cocultured Tcons and Treg cells were stimulated with (B and C) anti-CD3/CD28-coated Dynabeads or (D and E) soluble anti-CD3 with WT antigen-presenting cells (APCs). B and D, Counts per minute (CPM) in 1 representative experiment (*P = .011, **P = .0065, ***P = .0002, ****P < .0001); C and E, proportional suppression of CPM. A, n = 1; B and C, n = 3; D and E, n = 1. AREG, Amphiregulin.