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. 2016 Jun;137(6):1898–1902.e7. doi: 10.1016/j.jaci.2015.11.047

Fig 2.

Fig 2

Increased mRNA expression and IL-9 production in asthmatic patients with a positive skin test result. A,IRF4 mRNA expression analyzed by using quantitative real-time PCR in RNA isolated from whole blood (n = 13-14 children per group). B and C, Further subdivision of children shown in Fig 2, A, into healthy subjects with negative skin test results (n = 6, PreDicta, Fig 2, B; n = 104, Asthma BRIDGE, Fig 2, C) and asthmatic patients with positive skin test results (n = 8, PreDicta, Fig 2, B; n = 144, Asthma BRIDGE, Fig 2, C). D and E, PBMCs from the PreDicta children were cultured with PHA for 48 hours, and afterward, ELISA was performed for IL-9 production on cell supernatants (Fig 2, D: n = 19 healthy children and n = 20 asthmatic children; Fig 2, E: n = 8 healthy children with negative skin test results and n = 15 asthmatic children with positive skin test results). The Student t test was used to evaluate statistical significance. *P ≤ .05 and **P ≤ .01. Data are presented as means ± SEs.