Table 1.
Characteristics of antigens considered for recombinant expression and assessment in the murine L.donovani model.
| Name | Accession No. | Descriptor/Function | Fragment expressed | Size (kD) | Leishmania source | Leishmania homology | Human homolog (NP) | Identity to human (%) | comments | Other expression/adjuvant/delivery systems used | References |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MDH | XP_001682648.1 | malate dehydrogenase | full length | 33 | L. major | 97% L. infantum (310/321) 96% L. donovani (309/321) 93% L. mexicana (300/321) 88% L. panamensis (282/321) 88% L. braziliensis (281/321) |
00509.2 | 46 | 5 stretches >8aa, spread out equally | N/A | [24] |
| 8E | XP_001566870.1 | Heat shock protein | Δ509-661 | 17 | L. braziliensisa | 97% L. braziliensis (147/152) 98% L. panamensis (149/152) 82% L. infantum (124/152) 82% L. major (124/152) 80% L. mexicana (121/152) |
004125.3 | 36 | no stretches >5aa | N/A | [21] |
| H2B | XP_001682580.1 | Histone | full length | 12 | L. major | 99% L. infantum (106/107) 95% L. mexicana (102/107) 79% L. panamensis (84/107) 79% L. braziliensis (84/107) |
003511.1 | 43 | no stretches >6aa | protein with CpG ODN |
[22] |
| α tubulin |
XP_001681772.1 | Microtubule component | full length | 50 | L. major | 100% L. donovani 100% L. mexicana 100% L. major 99% L. braziliensis (449/451) |
116093.1 | 82 | very conserved | N/A | [24] |
| p45 | XP_001682593.1 | amino-peptidase | Δ20−380 | 40 | L. major | 99% L. donovani (358/361) 99% L. infantum (358/361) 99% L. mexicana (356/361) 93% L. panamensis (335/361) 92% L. braziliensis (333/361) |
006182 | 37 | 1 stretch of 9aa and 7aa (from aa62-85) | protein with BCG |
[23] |
| p21 | XP_003722922.1 | Unknown | full length | 21 | L. major | 97% L. infantum (186/191) 96% L. mexicana (184/191) 91% L. braziliensis (173/191) |
none | N/A | N/A | N/A | [24] |
| SMT | XP_001469832.1 | Sterol methyltransferase | full length | 40 | L. infantum | 99% L. donovani (352/353) 97% L. major (341/353) 93% L. mexicana (330/353) 86% L. braziliensis (302/353) 86% L. panamensis (302/353) |
none | N/A | N/A | protein with MPL-SE |
[30], [31] |
| Nh | XP_003860171.1 | Non-specific Nucleoside hydrolase | full length | 34 | L. donovani | 99% L. infantum (313/314) 96% L. major (300/314) 93% L. mexicana (293/314) 84% L. braziliensis (264/314) 84% L. panamensis (263/314) |
none | N/A | N/A | DNA | [32] |
| NS | XP_003860171.1XP_001469832.1 | Nh + SMT | full length full length | 74 |
L. infantum L. infantum |
see aboveb | chimeric difusion protein | protein with GLA-SE |
[27] | ||
| EPS |
XP_001467099.1 XP_001469316.1 XP_001469832.1 |
8E + p21 + SMT | Δ509−661 full length full length |
78 |
L. infantum L. infantum L. infantum |
see aboveb | chimeric trifusion protein | reported here | − |
a Sequence used was unique to the individual from whom this L. brasiliensis gene was isolated.
b See notes on each individual component.