Table 3.
| (a) Pyruvate–glucose interaction
| ||||||||
|---|---|---|---|---|---|---|---|---|
| Treatment | Concentration
|
Response
|
Mean angular response | Response (%) | ||||
| Pyruvate | Glucose | Drop number | ||||||
| 1 | 2 | 3 | 4 | |||||
| 1 | 3·16 × 10−4 M | 1 mg/ml | 4 | 5 | 6 | 4 | 38·95 | 39·5 |
| 2 | 3·16 × 10−4 M | 0 | 5 | 5 | 4 | 8 | 42·60 | 45·8 |
| 3 | 6·00 × 10−5 M | 1 mg/ml | 3 | 3 | 2 | 3 | 28·53 | 22·8 |
| 4 | 6·00 × 10−5 M | 0 | 0 | 0 | 1 | 0 | 10·43 | 3·3 |
| (b) Analysis of variance
| ||
|---|---|---|
| Source of variation | d.f | m.s. |
|
| ||
| Between drops | 3 | 9·122 |
| Between treatments | (3) | (832·208) |
| Pyruvate | 1 | 1814·760* |
| Glucose | 1 | 208·803 |
| P × G | 1 | 473·062† |
| Error | 9 | 36·551 |
| Total | 15 | |
| Theoretical variance | ∞ | 75·3 |
Response is the number of normal blastocysts from twelve 2-cell ova after 3 days cultivation in vitro.
Two-cell ova obtained 43 hr after HCG injection.
*
P< 0·001.
†
0·05 > P > 0·01.