Table 8.
| (a) Interaction between pyruvate, oxaloacetate and lactate at pH 7·38 and osmolarity 0·308
| |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Treatment | Pyruvate ×10−4M | Oxaloacetate ×10−4M | Lactate ×10−2M | Day 1 Response
|
Day 2 Response
|
Mean angular response | Response (%) | ||||||
| Drop number | Drop number | ||||||||||||
| 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | ||||||
| 1 | 5·0 | 5·0 | 5·0 | 3 | 1 | 2 | 5 | 8 | 6 | 7 | 10 | 40·81 | 43·75 |
| 2 | 5·0 | 5·0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 4 | 19·23 | 12·50 |
| 3 | 5·0 | 0 | 5·0 | 6 | 4 | 3 | 6 | 12 | 9 | 6 | 8 | 49·59 | 56·25 |
| 4 | 5·0 | 0 | 0 | 4 | 1 | 5 | 4 | 4 | 3 | 5 | 7 | 35·36 | 34·38 |
| 5 | 0 | 5·0 | 5·0 | 5 | 6 | 5 | 5 | 11 | 9 | 8 | 9 | 51·69 | 60·42 |
| 6 | 0 | 5·0 | 0 | 2 | 2 | 2 | 2 | 6 | 3 | 5 | 4 | 30·86 | 27·08 |
| 7 | 0 | 0 | 5·0 | 2 | 0 | 2 | 1 | 4 | 5 | 4 | 6 | 28·64 | 25·00 |
| 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8·30 | 0·00 |
| (b) Pyruvate × Oxaloacetate two-way table demonstrating interaction of effects (mean angular response)
| ||
|---|---|---|
| Oxaloacetate (M) | Pyruvate (M)
|
|
| 0 | 5 × 10−4 | |
|
| ||
| 0 | 18·47 | 42·48 |
| 5 × 10−4 | 41·28 | 30·02 |
| (c) Analysis of variance
| ||
|---|---|---|
| Source of variation | d.f. | m.s. |
|
| ||
| Between experiments | 1 | 4022·730* |
| Drops within experiments | 6 | 98·102 |
| Between drops | (7) | |
| Between treatments | (7) | |
| Pyruvate (P) | 1 | 650·250† |
| Oxaloacetate (O) | 1 | 428·490‡ |
| Lactate (L) | 1 | 5925·150* |
| P × O | 1 | 4973·775* |
| O × L | 1 | 61·623 |
| L × P | 1 | 28·623 |
| P × O × L | 1 | 47·266 |
| Experiments × treatments | 7 | 148·922 |
| Error | 42 | 51·711 |
| Total | 63 | |
| Theoretical variance | ∞ | 75·3 |
Response is the number of normal blastocysts from twelve 2-cell ova after 3 days cultivation in vitro. Two-cell ova obtained 43 hr after HCG injection. The standard error for the difference of two means in this example is 4·339.
*
P< 0·001.
†
0·05 > P> 0·01.
‡
0·01 > P> 0·001.