Table 9.
| (a) Interaction between Pyruvate and Lactate
| ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Treatment | Molar concentration
|
Day 1 Response
|
Day 2 Response
|
Mean angular response | Response (%) | |||||||
| Pyruvate × 10−4M | Lactate × 10−2M | Drop number | Drop number | |||||||||
| 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |||||
| 1 | 5·0 | 5·0 | 8 | 4 | 10 | 10 | 9 | 9 | 9 | 7 | 56·45 | 68·75 |
| 2 | 5·0 | 2·5 | 8 | 5 | 8 | 5 | 8 | 9 | 7 | 11 | 53·44 | 63·54 |
| 3 | 5·0 | 0 | 5 | 5 | 7 | 6 | 5 | 7 | 4 | 6 | 43·19 | 46·88 |
| 4 | 2·5 | 5·0 | 10 | 8 | 9 | 8 | 6 | 7 | 7 | 9 | 54·99 | 66·67 |
| 5 | 2·5 | 2·5 | 8 | 8 | 8 | 8 | 8 | 8 | 9 | 7 | 54·75 | 66·67 |
| 6 | 2·5 | 0 | 5 | 8 | 6 | 6 | 6 | 5 | 6 | 3 | 43·14 | 46·88 |
| 7 | 0 | 5·0 | 7 | 5 | 5 | 9 | 7 | 5 | 2 | 8 | 44·88 | 50·00 |
| 8 | 0 | 2·5 | 4 | 2 | 4 | 3 | 3 | 5 | 3 | 4 | 32·53 | 29·17 |
| 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8·30 | 0·00 |
| (b) Analysis of variance
| ||
|---|---|---|
| Source of variation | d.f. | m.s. |
|
| ||
| Between experiments | 1 | 2·961 |
| Drops within experiments | 6 | 65·249 |
| Between drops | (7) | |
| Between treatments | (8) | |
| Pyruvate (P) | ||
| linear (l) | 1 | 6052·521* |
| quadratic (q) | 1 | 1993·623* |
| Lactate (L) | ||
| linear (l) | 1 | 5073·797* |
| quadratic (q) | 1 | 413·106† |
| Pl × Ll | 1 | 1086·945* |
| Pl × Lq | 1 | 14·338 |
| Pq × Ll | 1 | 455·466† |
| Pq × Lq | 1 | 2·940 |
| Experiments × treatments | 8 | 70·095 |
| Error | 50 | 49·931 |
| Theoretical variance | ∞ | 75·3 |
Response is the number of normal blastocysts from twelve 2-cell ova after 3 days cultivation in vitro. Two-cell ova obtained 45 hr after HCG injection. The standard error for the difference of two means in this example is 4·339.
*
P< 0·001.
†
0·05 > P > 0·01.