Figure 5.

Influence of cj0031 on biofilm formation by Campylobacter jejuni strain NCTC11168. An assessment of biofilm formation was performed using phase variants and mutants of cj0031 in a chicken-adapted variant of C. jejuni strain 11168 (see Figure 4). Biofilm formation was measured by crystal violet (CV) staining of standing cultures in 24-well polystyrene plates following either 48 (panel A) or 72 (panel B) h incubation at 37°C under micro-aerobic conditions. Quantification of dissolved CV was made at 570 nm. Measurements represent six measurements determined from triplicate readings of two independent experiments. An unpaired Student's t-test was used to calculate P-values as compared to cj0031 ON (****P < 0.0001; and **P < 0.01).