Figure 3.

Pandan is a bimolecular miRNA sensor. (A) Absence of the target miRNA prevents duplexes P3 and P4 from forming, hence destabilizing the G-quadruplex and base triplet required for proper folding and binding of DFHBI. (B) Pairing between miRNA and sensor backbone in stem-loops P3 and P4 allows for stable complex folding with DFHBI. The arrow indicates the second unpaired nucleotide 3′ of SL P3, 5′ of SL P4, that in all our tested sensors was a Uracil (U). The GGGA partial transcriptional start site is boxed. Shading is consistent with Figure 1A–C. Based on Deigan-Warner et al. (20).