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. 2016 Mar 6;44(10):e92. doi: 10.1093/nar/gkw108

Figure 5.

Figure 5.

Parameters affecting selectivity of Pandan sensors. (A) Fluorescence intensity fold change of Pandan sensors for bantam-5p and miR-1000 miRNAs tested with target and non-target miRNAs. (B and C) Fluorescence intensity of Pandan sensors for bantam-5p, miR-263a and miR-1000 miRNAs tested with perfectly complementarity and sequence modified miRNAs. Panel B shows the effect of changing three residues in the miRNA 5′ end. Panel C shows the effects of single residue changes as a function of position in the miRNA. Sequence changes are shown in red. (D) Performance of the bantam-3p Pandan sensor with increasing fold excess of competing Drosophila total RNA, expressed as a percentage of raw fluorescence of sensor with bantam-3p in the absence of competing RNA. The presence of up to 1000-fold of competing RNA did not decrease the ability of bantam-3p sensor to detect bantam-3p (P > 0.05). Fluorescence experiments were performed in triplicate. Fluorescence intensity fold change shown is with respect to sensor + DFHBI-only controls (in absence of miRNA).