Table 1. Raw fluorescence readings in arbitrary units (a.u.) for each of the tested sensors in the absence and presence of 1 μM target miRNA.
Raw fluorescence (a.u) | ||||
---|---|---|---|---|
microRNA | # self-paired bases at 3′ end of SL P3 | Sensor + DFHBI alone | Sensor + DFHBI +miRNA | Fold change |
dme-bantam-5p | 6 | 395 | 46 464 | 118 |
dme-bantam-5p | 3 | 434 | 46 278 | 107 |
dme-miR-184 | 6 | 398 | 38 426 | 97 |
dme-bantam-3p | 3 | 457 | 43 279 | 95 |
hsa-miR21–5p | 3 | 425 | 29 018 | 68 |
hsa-let7f | 3 | 553 | 36 679 | 66 |
hsa-miR223–5p | 3 | 416 | 24 869 | 60 |
hsa-miR375 | 3 | 443 | 25 957 | 59 |
hsa-miR122 | 3 | 424 | 20 706 | 49 |
dme-miR-1 | 6 | 406 | 19 720 | 49 |
dme-miR-252 | 6 | 991 | 46 368 | 47 |
dme-miR-263a | 6 | 416 | 11 172 | 27 |
hsa-miR223–3p | 3 | 1518 | 20 756 | 14 |
dme-miR-1000 | 6 | 3343 | 26 788 | 8 |
dme-miR-263a | 3 | 437 | 2903 | 7 |
dme-miR-1000 | 3 | 5708 | 24 981 | 4 |
dme-miR-124 | 6 | 10 522 | 41 931 | 4 |
dme-miR-14 | 6 | 4708 | 14 175 | 3 |