Table 1. Plasmids designed and used in this study.
| Name | Elements | Description | Reference |
|---|---|---|---|
| pDF101 | PT7-SpAL-sTRSVac | ODF76 was PCR-amplified with ODF77 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF102 | PT7-SpAL-sTRSVinac | ODF76 was PCR-amplified with ODF78 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF103 | PT7-SpAM-sTRSVac | ODF76 was PCR-amplified with ODF79 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF104 | PT7-SpAM-sTRSVinac | ODF76 was PCR-amplified with ODF80 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF105 | PT7-SpAH-sTRSVac | ODF76 was PCR-amplified with ODF89 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF106 | PT7-SpAH-sTRSVinac | ODF76 was PCR-amplified with ODF90 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF107 | PT7-SpFL | ODF86 and ODF81 were annealed, filled in by PCR, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF109 | PT7-SpB | ODF88 and ODF81 were annealed, filled in by PCR, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF145 | PT7-SpAH-Env140ac | ODF150 and ODF151 were annealed, filled in by PCR, and PCR-amplified with ODF81 and ODF167, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF146 | PT7-SpAH-Env140inac | ODF152 and ODF153 were annealed, filled in by PCR, PCR-amplified with ODF81 and ODF168, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF148 | PT7-SpAH-Env140-C3ac | ODF154 and ODF155 were annealed, filled in by PCR, PCR-amplified with ODF81 and ODF167, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF151 | PT7-SpAH-Env140-H1ac | ODF156 and ODF157 were annealed, filled in by PCR, PCR-amplified with ODF81 and ODF167, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF168 | PT7-SpAH-sTRSV-L1.3ac | ODF185 was PCR-amplified with ODF89 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF169 | PT7-SpAH-sTRSV-L1.5ac | ODF93 was PCR-amplified with ODF89 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
| pDF179 | PT7-SpAH-sTRSV-L1.6ac | ODF184 was PCR-amplified with ODF89 and ODF81, restricted with EcoRI and XbaI and ligated into pUC57 (EcoRI/XbaI). | This work |
Abbreviations: Env140: engineered hammerhead ribozyme derived from environmental samples; ODFn: DNA oligonucleotide number n; PT7: Promoter recognized by phage T7 RNA-polymerase; SpAL/M/H: Split Spinach aptamer 5′ part with low (L), medium (M) or high (H) base-pairing strength; SpB: Split Spinach aptamer 3′ part; SpFL: Full-length Spinach aptamer; sTSRV: engineered hammerhead ribozyme derived from the natural ribozyme from the satellite RNA of the tobacco ringspot virus.