Figure 3.

Accelerated ribosome degradation in the S. aureus hpf null strain. Sucrose gradient profiles of S. aureus ribosomes harvested from TSB cultures at the logarithmic phase (top panels), the stationary phase (middle panels) and the late stationary phase (bottom panels). The y-axis corresponds to the absorbance at 254 nm of the ribosome species separated on a 5–25% sucrose gradient. Abs₂₅₄ values on the right serve as a ruler to compare relative ribosome peak height. Each panel corresponds to five Abs₂₆₀ units of total RNA input. The distribution of HPF_Sa was detected via immunoblotting. The 30S ribosomal subunit protein S7 is a marker control for fractionation. ‘(+)’ denotes loading control with the purified recombinant HPF_Sa.