Figure 3.

HPLC analysis of ESC-specific miRNA/pre-miRNA degradation in iPSC cytosol in the absence (left panels; miRNA only) or presence of DGG and TGG (right panels; miR + D/TGG). In the absence of glycylglycein protection, >46% and >73% of the whole miRNA/pre-miRNA population was quickly degraded (smeared down into smaller charges/sizes toward the left side of the HPLC chart) after incubating in iPSC cytosol for 12 and 24 h, respectively, whereas no degradation was detected when DGG/TGG and miRNAs/pre-miRNAs were mixed together in the cytosol.