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. 2016 Mar 21;44(10):4894–4906. doi: 10.1093/nar/gkw186

Figure 5.

Figure 5.

Functionality of DGG/TGG-protected siR-302. (A) Microinjection of DGG/TGG-mixed siR-302 into the cytoplasm of single keratinocytes that had been pre-transfected with an Oct4-promoter-driven green fluorescent protein transgene (Oct4-EGFP). The injected cells were labeled by co-injection of a red fluorescent dye—dextran texa red (DTR) with the DGG/TGG-mixed siR-302. (B) Western blot assays showed the gene silencing effects of siR-302 on reported miR-302-targeted genes, including lysine (K)-specific demethylase 1B (AOF1, KDM1b), methyl-CpG-binding protein 1, subunit p66 (MECP1-p66), methyl-CpG-binding protein 2 (MECP2), methyl-CpG-binding domain protein 2 (MBD2), DNA (cytosine-5-)-methyltransferase 1 (DNMT1) and nuclear receptor subfamily 2, group F, member 2 (NR2F2). In addition, silencing of these target genes led to the elevated expression of ESC-specific reprogramming factors, such as Oct4, Sox2 and Nanog.