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Purification of metaphase chromosomal-associated RNA. (A) Overview of the protocol. (B) Protein composition determined by SDS-PAGE after incubation with buffers containing the indicated increasing concentrations of salt. (C) Ultrastructure of metaphase chromosomes after incubation with the 0.2 M NaCl buffer. (D) Detection of β-actin mRNA using RT-PCR in different fractions of the sucrose gradient. (E) RT-PCR of U3 snRNA and β-actin in the mCARs (m) and cytoplasmic RNA (c) samples.
