Figure 1.

Principle of hybridization capture. A sequencing library containing targeted biomarkers is constructed and hybridized against a set of specific probes. Hybridization can be performed either in solution (solution hybrid selection, SHS) with biotinylated probes captured by streptavidin-coated magnetic beads, or on a solid support (array-based hybrid selection, AHS) on which probes are spotted. After hybridization, nontarget sequences are washed away, and the enriched sample is eluted and sequenced.