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. 2016 Jun 2;6:26834. doi: 10.1038/srep26834

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(a) Edible Plant Derived Exosome-like Nanoparticles (EPDENs) were purified from clarified cabbage lysate via ultracentrifugation. Levels of MIR2911 were quantified by qRT-PCR in various fractions: cabbage leaves liquefied in a blender (Liquefied Cabbage); Supernatant from 10,000 × g centrifugation (Slow Clarification); Supernatant from 150,000 × g ultracentrifugation (Ultra Clarification); EPDEN bands at 30% 45% interface (EPDEN band1) and 45% 60% interface (EPDEN band2) of sucrose gradient fractionation of total vesicles. (b) Size exclusion chromatography analysis of MIR2911 in ultracentrifugation-clarified cabbage lysate (c) qRT-PCR analysis of MIR2911 levels in proteinase K-treated or control (PBS-treated) ultracentrifugation-clarified cabbage lysate.