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. 2016 Jun 2;6:26834. doi: 10.1038/srep26834

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The stability of MIR2911, MIR168 and C7 were assayed in an in vitro digestion system that includes a gastric digestion phase and a intestinal digestion phase. Three different samples were digested: (a) cabbage lysate containing a measured amount of 10 pmoles of plant-derived MIR2911 and spiked-in 10 pmoles each of synthetic 2′ O-methylated MIR168 and C7; (b) 10 pmoles each of synthetic 2′ O-methylated MIR2911, MIR168 and C7; (c) 10 pmoles each of synthetic MIR2911, MIR168 and C7 without 2′ O-methylations. Aliquots of digestion reaction were removed at various time points for analysis: 30 min, 60 min in gastric phase, 5 min, 30 min, and 75 min in intestinal phase. Levels of surviving miRNAs were measured by qRT-PCR. The surviving percentages were calculated by comparing the levels of miRNAs at each time point to their starting levels before digestion.