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. 2016 Jun 2;6:27021. doi: 10.1038/srep27021

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Copyright © 2016, Macmillan Publishers Limited

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(A) Total DE, up- or down-regulated genes. (B) RT-qPCR validation of randomly selected up-regulated genes. (C) RT-qPCR of randomly selected down-regulated genes. Left panels in (B,C) show relative sequence read abundance (Integrated Genome Browser view) as histograms in WT, sr1-1 and SR1-YFP lines. The Y-axis indicates read depth with the same scale for all three lines. The gene structure is shown below the read depth profile. The lines represent introns and the boxes represent exons. The thinner boxes represent 5′ and 3′ UTRs. Right panels in (B,C) show fold change in expression level relative to WT. WT values were considered as 1. Student t-test was performed and significant differences (P < 0.05) among samples are labeled with different letters. The error bars represent SD.