Figure 3. Adding an overnight acclimation period to the hypothermic anaesthesia procedure increases the recovery proportion of a medaka disease model in the MR microscopy experiment.

(a) Typical water-temperature change during the entire hypothermic anaesthesia procedure. (b) Typical changes in water temperature (red) and room temperature (blue) in the final acclimation. (c) Typical temperature changes of the MR microscope during the imaging time. The temperature was measured just outside of the medaka imaging space near the gradient coils. The sudden changes at the beginning and the end correspond to the actions of placing the MR microscope probe in a magnet and pulling out the magnet, respectively. (d) The recovery proportion of the control Cab medaka was measured to be 1 and was significantly higher than that of the HFD Cab medaka, control P53 knockout medaka and HFD P53 knockout medaka in the MR microscopy experiment without the overnight acclimation (control Cab medaka: N = 18; HFD Cab medaka: N = 13; control P53 knockout medaka: N = 6; HFD P53 knockout medaka: N = 11). There was no statistical difference between the recovery proportions of the HFD Cab medaka, control P53 knockout medaka and HFD P53 knockout medaka. (e) Adding the overnight acclimation caused the combined recovery proportion of the HFD Cab medaka and the HFD P53 knockout medaka to be 1 (N_{w/overnight acclimation} = 6; HFD Cab medaka: N = 3; HFD P53 knockout medaka: N = 3), which was significantly higher than that of the HFD Cab medaka and the HFD P53 knockout medaka without the overnight acclimation (N_{w/o overnight acclimation} = 24; HFD Cab medaka: N = 13; HFD P53 knockout medaka: N = 11). Here, one-sided Fisher’s exact tests were used. ***P < 0.0001, *P < 0.05.