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. 2016 Jun 2;6:27091. doi: 10.1038/srep27091

Figure 1. LPS pretreatment protects mice from kidney I/R injury, which is negated by knockdown of miR-146a.

Figure 1

(a) Locked nucleic acid (LNA) anti-miR-146a (10 mg/kg) or anti-scrambled was administrated 2 hour prior to LPS injection. At 24 h after exposure to either normal saline (NS) or lipopolysaccharide (LPS; 3 mg/kg, intraperitoneal injection), mice were subjected to kidney ischemia surgery. miR-146a expression was examined and normalized to u6 at 6, 24, 48 h after kidney I/R by TaqMan-based real-time PCR, fold changes were calculated against the mean value of LPS + Sham group at each time point. (n = 6 mice per group for each time point. #P < 0.001, vs. LPS + I/R + Scrambled control, respectively. NS, not significant). (b) Serum creatinine was measured and data was expressed as mean ± SEM at 24 h after kidney I/R injury. (n = 6 mice per group, #P < 0.001 vs. LPS + I/R + Scrambled control). (c) Time course of serum creatinine levels after I/R in mice exposed to LPS with or without miR-146a knockdown. (n = 4 mice per group for each time point, *P < 0.05, #P < 0.001 vs. LPS + I/R + Scrambled control). (d) Representative periodic acid–Schiff (PAS)-stained kidney sections from mice receiving anti-miR-146a or anti-scrambled oligonucleotides at 24 h after pretreatment with either vehicle + I/R or LPS + I/R procedures (original magnification x200, Bar = 50 um. The black arrow indicates damaged tubules). (e) Abnormalities based on PAS-stained sections were graded by a semiquantitative histomorphological scoring system from 0 to 4. *P < 0.05 vs. LPS + I/R + Scrambled control.