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. 2016 Jun 2;6:27091. doi: 10.1038/srep27091

Figure 2. Proinflammatory cytokine (IL-1β and TNF-α) production in the LPS-challenged kidney following I/R injury.

Figure 2

(a) mRNA of IL-1β and TNF-α in the kidney medulla was determined by real-time PCR and was normalized to GAPDH at 6, 24, 48 h after kidney I/R. (n = 6 mice per group, **P < 0.01, #P < 0.001 vs. LPS + I/R + Scrambled control). (b) IL-1β and TNF-α protein levels at each time point were evaluated by Enzyme-linked immunosorbent assay (ELISA) at each time point. n = 6 mice/group, *P < 0.05, **P < 0.01, #P < 0.001 vs. LPS + I/R + Scrambled control). All data were expressed as mean ± SEM.