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. 2016 Jun 2;6:27091. doi: 10.1038/srep27091

Figure 6. Knockdown of miR-146a promotes TRAF6 and IRAK-1 protein expression.

Figure 6

LNA-anti-miR-146a or anti-scrambled oligonucleotides (10 m/kg) were administered 2 h before LPS treatment. After 24 h LPS exposure, the kidneys were subjected to I/R or Sham procedures followed by 6 h, 24 h, 48 h of reperfusion and then harvested. (a) mRNA of TRAF6 and IRAK-1 in the kidney medulla was determined by real-time RT-PCR and normalized to GAPDH at all time points. (n = 6 mice per group, P > 0.05 vs. LPS + I/R + Scrambled control group). (b) Kidney lysates of 6 h groups were subject to Western blot analysis for TRAF6 and IRAK-1. (c) The Western blots from all 6 h experiments were quantified by densitometry analysis. The ratios of TRAF6/ β-actin and IRAK-1/ β-actin were calculated; the fold changes relative to LPS + Sham protein are shown. (n = 6 mice per group, *P < 0.05, #P < 0.001, vs. LPS + I/R + Scrambled control group). (d) Time course of TRAF6 and IRAK-1 expression was confirmed by Western-blot in the LPS-challenged kidney following I/R injury.