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. Author manuscript; available in PMC: 2017 May 19.
Published in final edited form as: Cell Chem Biol. 2016 May 12;23(5):618–628. doi: 10.1016/j.chembiol.2016.04.006

Figure 5. The rod domain of peripherin is recognized by autoantibodies in peripherin-reactive sera.

Figure 5

A) Schematic representation of the GST fusion proteins used to determine the antibody-binding domain. Each construct was mounted onto the surface of 10 μm TentaGel microspheres via glutathione, which was covalently attached to the microspheres to perform binding competition assays using the flow cytometry assay. See also Figure S6 and Table S5. B) Serum competition binding analysis using the coiled coil domain-GST fusion protein as a capture agent. C) Serum competition binding analysis using the head/tail domain-fusion protein as a capture agent.