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. 2016 Jun 2;6:27255. doi: 10.1038/srep27255

Figure 5. Calcium salts, phosphates, and serum determine the formation of the self-propagating CPB.

Figure 5

(a) An increase in the concentration of calcium salts and phosphates promotes the formation of CPB both at 4 and 37 °C. (b) A decrease in the fetal bovine serum concentration also enhances this process both at 4 and 37 °C. (c) The ratio of calcium salts to phosphates does not affect the formation of CPB both at 4 and 37 °C. (d) Both natural (red) and artificial (green) CPB are able to self-propagate. (e) DLS particle concentration analysis of CPB cultured at 4 °C for 7 days. (f) DLS particle size distribution analysis of CPB cultured at 4 °C for 7 days. All experiments were performed in triplicate.