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. 2016 Jun 2;6:27206. doi: 10.1038/srep27206

Figure 1. Immunostimulation and maturation of BMDCs with cationic liposomes.

Figure 1

Cultured bone-marrow derived DCs (day 6) were stimulated with only media, LPS (1μg/ml) and cationic (CL) liposomes (100 μM liposome concentration) for 24 h. (a) Afterwards cells were immunostained with antibodies against CD11c as a dendritic cell marker and assessed for expression of the co-stimulatory molecules CD80, CD86 and CD83 by flow cytometry. For the analysis, CD11c+ cells were gated first before gating on the selected markers. Numbers inside the histogram represent MFI values and percent positive cells. Corresponding graph in right panel summarizes the MFI values of three experiments. *p < 0.05, ***p < 0.001 analyzed by one-way ANOVA, followed by Tukey’s multiple comparison test, compared to untreated control. (b) Stimulated dendritic cell culture supernatants were removed at 48 h for IL-12p40 quantification and nitric oxide (NO) production. Data represent triplicate mean ± SE. ***p < 0.001 analyzed by one-way ANOVA, followed by Tukey’s multiple comparison test, compared to untreated control.