Figure 4. Induction of CD8⁺ T cells in vivo by cationic liposome encapsulated antigen.

(a) Schematic outline of the experimental protocol. (b) Groups of five mice were injected subcutaneously with PBS, empty cationic liposomes (CL), 2.5 μg rgp63 (rgp) alone or encapsulated in cationic liposomes (lrgp). Animals were boosted on day 14 with the same antigen preparations received initially and 5 days later draining lymph nodes (dLN) were removed. CD8⁺ T cells from dLN suspensions were purified magnetically using MACS. 1 × 10⁵ CD8⁺ T cells were incubated with 1 × 10⁴ BMDCs (10:1 ratio) that had been incubated with liposomal rgp63 (2.5 μg/ml) for 18 h. T cell proliferation was measured on day 4. Data represent triplicate mean CPM × 10³ ± SE. **p < 0.01 analyzed by one-way ANOVA followed by Tukey’s multiple comparison tests.