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. 2016 Jun 2;6:27221. doi: 10.1038/srep27221

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Copyright © 2016, Macmillan Publishers Limited

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Samples were labeled with rhodamine-phalloidin (left panels and magenta in right-panel merges) and anti-ANXA5 (middle panels and green in right-panel merges). (a) In P7 saccule, hair bundles of all hair cells are labeled uniformly. (b) In P28 saccule, differences between neighboring bundles are apparent, with shorter bundles having higher ANXA5 immunoreactivity. (c) Relationship between the length of the tallest stereocilium in a saccular bundle and the ANXA5 fluorescence signal (AU, arbitrary units). Data were fit with a logistic function: y = 64−53/(1 + e^−(x−8.6)). (d) High-magnification views of several hair cells with short bundles. Labeling is strong at stereocilia tips. Panel full widths: a, 180 μm; b, 130 μm; d, 20 μm.