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. 2016 May 24;7:11657. doi: 10.1038/ncomms11657

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(a) tRNA^fMet_CGG variants bearing D-arm substitutions. Bases that differ from the wild-type tRNA^fMet are highlighted in red. (b) Translation of mR2 mRNA using the Pro-tRNA^fMet_CGG variants. Relative translation yields of the P2 peptide are shown. See Supplementary Fig. 3n for absolute peptide yields. (c) tRNA^fMet_CAU variants with D-arm substitutions. (d) fMet–Pmn formation using f[³⁵S] Met-tRNA^fMet_CAU variants. Error bars, s.d. (n=3). See Supplementary Fig. 3o for the yields of fMet–Pmn.