Table 2.

2,3-Butanediol (2,3-BD) production by native or engineered Bacillus strains

Strain	Substrate	2,3-BD isomer	Method	Concentration (g/L)	Yield (g/g)	Productivity (g/L–h)	References
Bacillus subtilis
AJ 1992 (WT)	Glucose	Mixture	Batch	2.5	0.38	0.33	[36]
RB03a	Glucose	Mixture	Batch	6.1	0.34	0.41	[37]
Bacillus amyloliquefaciens
F (WT)	Glucose	Mixture	Batch	33.0	0.33	–	[9]
B10-127 (WT)	Glucose	Mixture	Shake-flask	55.8	0.40	0.80	[38]
			Fed-batch	92.3	0.15	0.96
GARb	Crude glycerol	Mixture	Fed-batch	102.3	0.44	1.16	[39]
Bacillus licheniformis
Wild-type strain	Glucose	Mixture	Static flask	8.7	0.47	0.12	[40]
BL5c	Glucose	D-2,3-BD	Batch	12.2	0.45	2.65	[41]
WX-02ΔbudC	Glucose	D-2,3-BD	Shake-flask	30.8	0.27	1.28	[13]
X10 (WT)	Concentrated corn stover hydrolysate	Mixture	Fed-batch	74.0	0.47	2.06	[42]
DSM 8785 (WT)	Glucose	D-2,3-BD	Batch	72.6	0.42	0.86	[43]
			Fed-batch	144.7	0.40	1.14
			Immobilized	118.3	0.41	1.14
WX-02ΔgdhΔacoR	Glucose	Meso-2,3-BD	Shake-flask	28.2	0.24	1.18	This work
			Batch	35.1	0.35	1.30	This work
			Fed-batch	98.0	0.40	0.94	This work

^a B. subtilis 168 containing pAL10abA (P_alsSD-bdhA)

^b B. amyloliquefaciens B10-127 with pMA5-acr-HapII-dhaD-P_bdhA-alsR

^c B. licheniformis BL1Δldh