Table 2. Kinetic parameters (V_max and K _m) for [³H]dopamine uptake by WT hDAT and its mutants.

	WT hDAT	H547A-hDAT	H547P-hDAT	Y470H-hDAT
Vmax (pmol/min/105 cells)	12.4 ± 1.51	36.7 ± 4.12*	0.2 ± 0.09*	2.2 ± 0.56*
Km (μM)	1.3 ± 0.46	3.6 ± 1.47	2.6 ± 1.58	1.5 ± 0.08

Data are presented as mean ± S.E.M. values from five to seven independent experiments performed in duplicates. *p < 0.05 compared with WT hDAT (unpaired Student’s t test).