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. 2016 Jun 2;6:27331. doi: 10.1038/srep27331

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(a) The survival rate of the animals. (b) Tumor volumes in mm³ of DYT-40-treated and Temozolomide (TMZ)-treated animals compared with vehicle-treated animals. (c) A magnification of the striatum with a representative part of the tumor by H&E staining. DYT-40-treated animals showed smaller tumor volumes compared to untreated control animals (Scale bar: 1 mm, upper). The DYT-40-treated rats show less histopathological changes of the malignant C6 glioma compared to untreated animals (Scale bar: 100 μm, below). (d) Reduced AEG-1 expression and increased apoptosis caused by DYT-40 administration. Ten-micrometer thick cryostat sections are obtained and stained for AEG-1 and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL), and counterstained with hematoxylin.