Table 1. Infectivity scoring of Tomato leaf curl New Delhi virus-infiltrated control, mock and SlRPT4 silenced plant.
Plant | Plant infected/inoculated | Severe symptom appearance | Symptom severitya | Over all gradeb |
---|---|---|---|---|
H-88-78-1 (HT) | 5/75 | 14 | + | T |
Mock (HTRV:00+T) | 7/75 | 13 | + | T |
SlRPT4 silenced (HTRV:SlRPT4+T) | 51/75 | 7 | ++++ | HS |
Mock (HTRV:00) | – | – | – | – |
SlRPT4 silenced (HTRV:SlRPT4) | – | – | – | – |
Infectivity indexing was done on systemic leaves of experimental plants at 21 days post-inoculation, following the protocol described elsewhere21. Briefly, a) +, Least severe; ++, moderately severe; +++, severe; ++++, highly severe. b) T, Tolerant (1–20%); MT, Moderate tolerant (20.1–40%); S, Susceptible (40.1–60%); HS, Highly susceptible (60.1–100%). ‘–’ represent non-ToLCNDV infected samples.