Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Optimized Chromatographic Conditions
| Instrumentation | Optimized chromatographic conditions |
|---|---|
| Equipment | RP-HPLC Waters 2695 equipped with auto PDA detector |
| Column | Symmetry ODS 18 (250×4.6 mm, 5 µm) |
| Mobile phase | Phosphate buffer (pH 2.5) and ACN in the ratio of 55:45% v/v |
| Wavelength | 250 nm |
| Flow rate | 1 mL/min |
| Injection volume | 10 µL |
| Runtime | 10 min |
