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. Author manuscript; available in PMC: 2016 Jun 2.
Published in final edited form as: Chem Res Toxicol. 2014 Feb 13;27(4):674–682. doi: 10.1021/tx500011m

Figure 1.

Figure 1

Hydralazine-mediated cleavage (A) in pHOT1 DNA following oxidation with Cu2+ (25 μM). The DNA cleavage was examined as described in the Methods section. Lane 1: DNA in the presence of Cu2+ alone; Lane 2: Hydralazine (1000 μM) and Cu2+; Lane 3: Hydralazine (500 μM) and Cu2+; Lane 4: Hydralazine (250 μM) and Cu2+; Lane 5: Hydralazine (125 μM) and Cu2+; Lane 6: Hydralazine (50 μM) and Cu2+; and Lane 7: Hydralazine (25 μM) and Cu2+. (B) Effects of scavengers on the DNA cleavage induced by hydralazine (250 μM) and Cu2+ (25 μM). Scavengers were incubated with hydralazine-DNA complexes for 5–10 min before adding Cu2+. Lane 1: control DNA; Lane 2: hydralazine in the presence of Cu2+; Lane 3: reduced GSH (1000 μM); lane 4: SOD (50 U/incubation); Lane 5: catalase (2500 U/incubation); Lane 6: SOD and catalase; Lane 7: DMPO (100 mM) and Lane 8: DMSO (1 mM).