Figure 2.
Detection of 1O2 from hydralazine (125 uM) in the presence of Cu2+ (25uM) and TEMP (100 mM). a: complete System; b: Hydralazine alone; c: Cu2+ alone and d: in the presence of Rose Bengal (20 uM) and light for 5 min. No signal for the nitroxide was detected in the absence of light with Rose Bengal. The ESR studies were carried out as described in the Methods section; a–c: conversion time = time constant = 163 ms. d: 81 ms.