Skip to main content
. Author manuscript; available in PMC: 2016 Jun 2.
Published in final edited form as: Chem Res Toxicol. 2014 Feb 13;27(4):674–682. doi: 10.1021/tx500011m

Figure 3.

Figure 3

Oxygen consumption (A) with different concentrations of hydralazine in the presence of Cu2+ (50 μM). The oxygen consumption was carried out as described in the Methods section. Tracing 1: 125 μM hydralazine; Tracing 2: 50 μM hydralazine; Tracing 3: 250 μM hydralazine; Tracing 4: 500 μM hydralazine; and Tracing 5: 1000 μM hydralazine. (B) Effects of oxy-radical scavengers on oxygen consumption from 125 μM hydralazine and 50 μM Cu2+. Tracing 1: control; Tracing 2: 100 mM DMPO; Tracing 3: catalase (6000 U/ml); Tracing 4: SOD (500 U/ml); and Tracing 5: 1000 μM GSH. (C) Effect of DNA (200 μg/ml) on the oxygen consumption by various concentrations of hydralazine in the presence of Cu2+ (50 μM). Tracing 1: 250 μM hydralazine; Tracing 2: 125 μM hydralazine; Tracing 3: 500 μM hydralazine; Tracing 4: 1000 μM hydralazine and Tracing 5: 50 μM hydralazine. DNA was added to the hydralazine solutions and incubated for 10 min before adding Cu2+ and measuring the O2 consumption.