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. 2016 Jun 2;16:20. doi: 10.1186/s12861-016-0121-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Fluorescence microscope-, confocal-, and gSTED images of PNA labelled Macrostomum lignano. a Overview of a PNA labelled adult animal. Asterisks indicate position of eyes. b Tail plate of a hatchling (confocal projection) with labelled adhesive gland cells. (c-d2) Projections of gSTED images. c Adhesive gland cell bodies with labelled vesicles in the cytoplasm, dark areas represent the nucleus. (d1) Gland cell necks filled with vesicles and (d2) detail thereof. Inset 1: detail of stained vesicles, arrowheads indicate the circular staining surrounding an unstained center. Inset 2: TEM image of adhesive gland vesicles, arrowhead indicates the lucid rim surrounding an electron-dense core. Acb adhesive gland cell bodies, acn adhesive gland cell necks, fg frontal glands, nu nucleus, ph pharyngeal glands, tp tail plate. Scale bars: (A) 100 μm, (B) 10 μm, (C,D2) 2 μm, (D1) 5 μm, (D inset) 200 nm