Fig. 3.

Schematic drawing and labelling of Macrostomum lignano adhesive organs. a Schematic drawing of adhesive organs at the tail plate. Note that the microvilli collar, protruding from the epidermis do not contain any intermediate filaments but actin filaments. (b1-3) Anti-Macif1 antibody staining of a whole specimen. Arrowhead indicates intermediate filaments in the anchor cells. (c1-3) Confocal projection of PNA- and antibody Macif1 double staining (boxed area in C’). (c1) PNA labelled adhesive gland cell bodies and -necks. (c2) Intermediate filaments in the anchor cells. Arrowhead indicates intermediate filament connections to the extracellular matrix. (c3) Overlay of PNA lectin and Macif1 staining. Note that the adhesive gland cell necks are surrounded by intermediate filaments of anchor cells. Arrow highlights adhesive gland cell necks at the area of microvilli collar. Acb adhesive gland cell bodies, acn adhesive gland cell necks, if intermediate filaments, ph pharynx. Scale bars: (B) 100 μm, (C1-3) 20 μm, (C1’-3’) 10 μm