Table 1.
Lectin labelling of different cells and tissues in Macrostomum lignano
| Lectin | Acronym | Number specimen | Overall | Epi-dermis | Adhesive glands | Frontal glands 1, 2, 4 | Frontal glands 3 | Pharyngeal glands | Testes | Developing eggs | Cement glands | Antrum | Prostate |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Lens culinaris agglutinin | LCA | n = 25 | +++a | ||||||||||
| Phaseolus vulgaris erythro agglutinin | PHA-E | n = 28 | +++b | ||||||||||
| Phaseolus vulgaris leuco agglutinin | PHA-L | n = 27 | +++b | ||||||||||
| Succinylated wheat germ agglutinin | sWGA | n = 17 | +++ | ++ | ++ | ||||||||
| Griffonia (Bandeiraea) simplicifolia lectin I | GSL I | n = 23 | +++ | ++ | +++ | +++ | |||||||
| Soybean agglutinin | SBA | n = 45 | +++ | ++ | +++ | + | +++ | ++ | |||||
| Peanut agglutinin | PNA | n = 48 | +++ | +++ | +++ | + | ++ | ++ | +++ | ||||
| Ricinus communis agglutinin | RCA | n = 24 | ++ | +++ | ++ | +++ | +++ | +++ | |||||
| Concanavaline A | Con A | n = 32 | +++ | ||||||||||
| Wheat germ agglutinin | WGA | n = 23 | ~ | ||||||||||
| Sambucus nigra agglutinin | SNA | n = 16 | ~ | ||||||||||
| Maackia amurensis lectin II | MAL II | n = 18 | ~ | ||||||||||
| Dolichos bilforus agglutinin | DBA | n = 21 | ~ | ||||||||||
| Sophora Japonica agglutinin | SJA | n = 20 | ~ | ||||||||||
| Ulex europaeus agglutinin 1 | UEA 1 | n = 12 | ~ | ||||||||||
| neg. control (without lectin) | n = 36 | ~ |
+weak labelling,++ intermediate labelling, +++ strong labelling, ~ unspecific background
aprobably epidermal cell junctions
bglycocalyx of epidermal microvilli and modified microvilli of anchor cells