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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Cell Signal. 2015 Nov 11;28(2):7–22. doi: 10.1016/j.cellsig.2015.11.005

Figure 10. Effects of TLR3 ligands on HIV-1 infection of MDM.

Figure 10

Human MDM were infected with the macrophage tropic HIV-1ADA, with or without PIC (10, 25, or 50 μg/ml) in the media. Culture supernatants were collected every 2 or 3 days from day-5 to day-18 post-infection (A–F) and viral replication was estimated by quantifying reverse transcriptase activity. For some experiments, infected MDM were treated with PIC only during the 4h initial viral exposure and subsequent cultures performed without PIC in the media (“T”); for other experiments, infected MDM were treated with PIC throughout the 5 to 18 days experiments (“C”). From day-5 to day-18 p.i., PIC decreased HIV-1 infection in a dose-dependent manner, with higher inhibition observed in infected MDM continuously cultured with media containing PIC (“C”). ***P<0.001, **P<0.01. Data shown are representative data from 3 independent experiments, using MDM from 3 different donors, with each donor tested in triplicate.