Table 1. Statistics for RNA-Seq based sequencing, assembly and functional annotation for Microhyla fissipes.

Sequencing results	Number of total raw reads	184,817,024
Assembling results	Number of total clean reads	177,585,214
	Number of unigenes	110,061
	Total length (nt) of total unigenes	80,600,119
	Mean length (nt) of total unigenes	732
	N50 (nt) of total unigenes	1330
Annotation	Unigenes with Nr	27,267 (24.77% of 110,061 unigenes)
	Unigenes with Nt	12,905 (11.72%)
	Unigenes with Swiss-Prot	23,293 (21.16%)
	Unigenes with KOG	13,459 (12.22%)
	Unigenes with PFAM	25,035 (22.74%)
	Unigenes with GO	26,692 (24.25%)
	Unigenes with KEGG	12,134 (11.02%)
	Total unigenes annotated	34,938 (31.74% of 110,061 unigenes)