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. Author manuscript; available in PMC: 2016 Jun 2.
Published in final edited form as: Free Radic Biol Med. 2015 Jul 21;89:379–386. doi: 10.1016/j.freeradbiomed.2015.07.017

Figure 5. SOD2-null HEK293T cells displayed significant alterations in mitochondrial bioenergetics.

Figure 5

(A) Real-time oxygen consumption rate (OCR; pmol/min) was measured with the Seahorse Extracellular Flux Analyzer. A representative trace from a mitochondrial stress test is shown comparing OCR of wild-type cells (closed circles) to knock-out cells (open circles) with arrows indicating sequential additions of oligomycin, FCCP, and antimycin A/rotenone.

(B) Quantification of OCR (pMol/min/µg protein) in wild-type and SOD2-null cells. *P<0.01 compared to wild-type.

(C) Quantification of extracellular acidification rate (ECAR; mpH/min/µg protein) in wild-type and SOD2-null cells. *P<0.01 compared to wild-type.

(D) A 3.5-fold decrease in mitochondrial reserve capacity was detected in knock-out cells. *P = 0.01 compared to wild-type.

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