Skip to main content
. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Anal Chem. 2015 Sep 4;87(19):9855–9863. doi: 10.1021/acs.analchem.5b02307

Figure 2.

Figure 2

Structure and reporter ion deconvolution for a CMT sixplex isobaric labeling reagent set. (A) (Left) CMT NHS activated ester structure. Stars indicate all positions where heavy isotopes are incorporated across any of the 6-plex reagents A–F. (Right) Primary and secondary reporter ion structures and masses of the CMT 6-plex reagents used in this study. Individual 6-plex reagents are denoted with the letters A–F. While multiple reagents share overlapping reporter ions, each of A–F can be distinguished by their unique combinations of primary and secondary reporter ions. (B) Expected reporter ion intensities of a sample mixed with equal amounts of CMT 6-plex reagents A–F. Both the primary and the secondary reporter ion series contain 100% of the sample mixing information. Colors correspond to those used in (A). (C) The fraction of the reporter ion intensity originating from each CMT 6-plex reagents A–F is calculated from a series of linear equations. (D) Actual mass spectrum of reporter ion distribution of a 1:1:1:1:1:1 mixture of a CMT 6-plex labeled peptide.