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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Anal Chem. 2015 Sep 4;87(19):9855–9863. doi: 10.1021/acs.analchem.5b02307

Figure 5.

Figure 5

Utilization of multiple reporter ion series allows for rapid expansion of isobaric multiplexing capacity. (A) CMT reagents produce three series of reporter ions simultaneously under HCD conditions, as exemplified by the HCD spectrum of CMT reagent E free acid (NCE = 35). Only the largest two of these reporter ion series are isotopically encoded in the current application of the reagent. (B) Comparison of the multiplexing capacity of TMT and CMT reagents utilizing either two or three reporter ion series. Reagent sets incorporating 15N are limited to 1 such instance per reagent within the set.