Fig. 1.

Chlamydia infection causes elevated levels of sortilin. (A) Whole cell lysates of C. trachomatis L2-infected HeLa cells were harvested at indicated time points. 20 μg of proteins were subjected to western blot using rabbit polyclonal anti-sortilin and mouse monoclonal anti-beta actin antibodies followed by IR dye-conjugated secondary antibodies. Fluorescent intensities were detected by Li-Cor Odyssey Infrared Imaging System and (B) quantified using FIJI demonstrating a stark increase sortilin levels during infection. Error bars denote mean±s.e.m., N=2; one-way ANOVA; *P<0.05, **P<0.005.