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. 2016 May 27;8:117–124. doi: 10.2147/BCTT.S106097

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© 2016 Isfoss et al. This work is published and licensed by Dove Medical Press Limited

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ALDH1⁺ cells in TDLUs.

Notes: Fluorescence microscope images of a triple-labeled TDLU from benign breast tissue with the following color coding: (A) DAPI nuclear staining (blue); (B) ALDH1 (green); (C) CD44 (white); (D) CD24 (red). (E) A digitally composed image showing all channels merged. Most of the cells located basally in ductules are ALDH⁻ CD44⁺, whereas some adluminal cells are ALDH1⁺ CD44⁺ and none are CD24⁺. In stroma, elongated ALDH1⁺ cells are seen between the ductules, and many such cells are present in the junction between TDLU stroma and generic connective tissue of the breast (arrows). Scale bars = 20 µm.

Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; TDLUs, terminal duct-lobular units.